diff --git a/src/python/mraapy.c b/src/python/mraapy.c
index a3536f0..c1f8521 100644
--- a/src/python/mraapy.c
+++ b/src/python/mraapy.c
@@ -25,9 +25,13 @@ mraa_python_isr(void (*isr)(void*), void* isr_args)
     if (arglist == NULL) {
         syslog(LOG_ERR, "gpio: Py_BuildValue NULL");
     } else {
+#if PY_VERSION_HEX >= 0x03090000
+        ret = PyObject_CallObject((PyObject*) isr, arglist);
+#else
         ret = PyEval_CallObject((PyObject*) isr, arglist);
+#endif
         if (ret == NULL) {
-            syslog(LOG_ERR, "gpio: PyEval_CallObject failed");
+            syslog(LOG_ERR, "gpio: Python call failed");
             PyObject *pvalue, *ptype, *ptraceback;
             PyObject *pvalue_pystr, *ptype_pystr, *ptraceback_pystr;
             PyObject *pvalue_ustr, *ptype_ustr, *ptraceback_ustr;
diff --git a/src/python/mraapython.i b/src/python/mraapython.i
index 521ef53..3a85d26 100644
--- a/src/python/mraapython.i
+++ b/src/python/mraapython.i
@@ -151,7 +151,13 @@ class Spi;
     // Initialise python threads, this allows use to grab the GIL when we are
     // required to do so
     Py_InitializeEx(0);
+#if PY_VERSION_HEX < 0x03090000
+    // PyEval_InitThreads() is deprecated since Python 3.9 and removed in Python 3.12
+    // In Python 3.7+, the GIL is initialized automatically by Py_Initialize()
+    // Only call PyEval_InitThreads() for Python < 3.9
+    // Reference: https://docs.python.org/3/c-api/init.html#c.PyEval_InitThreads
     PyEval_InitThreads();
+#endif
     // Add mraa_init() to the module initialisation process and set isr function
     mraa_result_t res = mraa_init();
     if (res == MRAA_SUCCESS) {